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Image Search Results
Journal: Frontiers in Immunology
Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice
doi: 10.3389/fimmu.2020.00712
Figure Lengend Snippet: In vivo detection of the red fluorescent protein in different organs of vaccinated NOD mice. NOD mice were given a single oral dose of Salmonella -containing plasmid (pSBbi-RP/SB100, n = 10) compared to Salmonella only ( n = 10). Four weeks post-vaccination, red fluorescent protein was detected in different organs. (A) Representative FACS plots gated the frequency of live red-labeled CD11c + and CD11c – cells in the spleens, pancreatic lymph nodes (PLN), mesenteric lymph nodes (MLN), and Peyer’s patches (PP) of mice from each group. (B) Quantification of the percentages of the CD11c + and CD11c – red-labeled cells in all organs. (C) Quantification of the percentages of red-labeled CD11c + MHCII + in total live cells isolated from the spleen, PLN, MLN, and PP. The data shown are as the average with standard deviation (SD) obtained from two independent experiments. Statistical analysis using two-way ANOVA shows significance between groups (**** p < 0.0001).
Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using
Techniques: In Vivo, Plasmid Preparation, Labeling, Isolation, Standard Deviation
Journal: Frontiers in Immunology
Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice
doi: 10.3389/fimmu.2020.00712
Figure Lengend Snippet: Vaccine-delivered TGFβ alters levels of DCs in secondary lymphoid tissues. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100, n = 10) or Salmonella (pSBbi-RP/SB100, n = 10). Four weeks post-vaccination, red fluorescent protein was detected in DCs of different organs. (A) Representative FACS plots gated the frequency of MHCII + CD11c + Td-Tomato + cells in the spleens, PLN, MLN, and PP of mice from each group. (B) Quantification of the percentages of MHCII + CD11c + Td-Tomato + cells in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (*** p < 0.001, **** p < 0 0001).
Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using
Techniques:
Journal: Frontiers in Immunology
Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice
doi: 10.3389/fimmu.2020.00712
Figure Lengend Snippet: Vaccine-delivered TGFβ alters levels of gut DCs. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100, n = 10) or Salmonella (pSBbi-RP/SB100, n = 10). Four weeks post-vaccination, red fluorescent protein was detected in DCs of different organs. (A) Representative FACS plots gated the frequency of the live red-labeled MHCII + CD11c + CD103 + cells in the spleens, PLN, MLN, and PP of mice from each group. (B) Quantification of the numbers of MHCII + CD11c + CD103 + in red-labeled cells in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (**** p < 0.0001).
Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using
Techniques: Labeling
Journal: Frontiers in Immunology
Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice
doi: 10.3389/fimmu.2020.00712
Figure Lengend Snippet: Endogenous TGFβ alters PDL-1 and CTLA4 expression in secondary lymphoid tissues. NOD mice were given a single oral dose of Salmonella (pSBbi-RP-TGFβ/SB100) or Salmonella (pSBbi-RP/SB100). Four weeks post-vaccination, the fold changes in gene expression of (A) CD274 (PDL-1) in sorted CD11c cells and (C) CTLA4 in sorted CD4 cells isolated from specified organs was determined. Percentage of CD11c + cells expressing PDL-1 (B) or CD4 + cells expressing CTLA4 (D) were quantified in the spleen, PLN, MLN, and PP. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between Salmonella (pSBbi-RP-TGFβ/SB100) and Salmonella (pSBbi-RP/SB100) groups (** p < 0.01, *** p < 0.001, **** p < 0 0001).
Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using
Techniques: Expressing, Gene Expression, Isolation
Journal: Frontiers in Immunology
Article Title: Tracking of an Oral Salmonella -Based Vaccine for Type 1 Diabetes in Non-obese Diabetic Mice
doi: 10.3389/fimmu.2020.00712
Figure Lengend Snippet: A Salmonella -based vaccine increase DCs in lymphoid organs. NOD mice were injected with 2.5 μg/mouse of anti-CD3 mAb for 5 days followed by 2 doses of oral vaccine containing PPI + TGFβ + IL10 ( n = 5). Four weeks post-vaccination, DCs were analyzed in different organs by FACS analysis. (A) Quantification of the percentage of CD11c + in total live cells of the spleen, PLN, MLN, and PP. (B) MLN, (C) PP, (D) PLN, and (E) spleen expression of CD80, CD86, and MHCII was measured in DCs from each organ. The data shown are the average with SD from two independent experiments. Statistical analysis using two-way ANOVA shows significance between vaccine and vehicle treated groups (* p < 0.05, ** p < 0.01, **** p < 0.0001).
Article Snippet: Dendritic cells (DCs) were isolated from pooled splenocytes (5 mice) isolated from either vaccinated or non-vaccinated mice using
Techniques: Injection, Expressing